Data upload is typically the most challenging step of using ExpressAnalyst for raw data processing, since FASTQ files are extremely large and must be uploaded over a network connection. The upload time will depend on both your file size and your network speed. In our own tests on a laptop and home wifi network, it took ~30 minutes to upload one FASTQ file that was ~2.5GB.
If your data size is very large or internet speed is very slow, we strongly suggest you to install the Docker image of ExpressAnalyst to process raw RNAseq data locally. The resulting count table can be uploaded to ExpressAnalyst for further statistical and functional analysis